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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene continues to be noticed in many tumors, which ends up in chromosome instability, and so may possibly add to tumorigenesis. This protein was identified to affiliate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a vital job in advertising mobile-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, are described. Two added splice variants were being noted but specific nucleotide sequence facts isn't nevertheless out there.
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Blocking peptides are peptides that bind specifically to the concentrate on antibody and block antibody binding. These peptide usually incorporates the epitope regarded via the antibody.
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This guidebook outlines even further probable optimization of mobile and immunoassay parameters to make sure the best possible final results are...
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This tutorial outlines further feasible optimization of cellular and immunoassay parameters to make certain the absolute best benefits are...
Antibodies certain to the blocking peptide no more bind to the epitope around the goal protein. By comparing the staining in the blocked antibody compared to the antibody alone, one can see which staining is specific.
Target Info Cyclin E belongs towards the very conserved cyclin family members, whose members are characterised by a spectacular periodicity in protein abundance with the cell cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation styles which lead towards the temporal coordination of each and every mitotic function. Cyclin E types a posh with and features like a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S transition. This protein accumulates for the G1-S stage boundary and is degraded as cells development by means of S phase.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...
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